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Advances in Crop Science and Technology

.: Home > Advances in Crop Science and Technology > 2014 > Volume 2 Number 3 > Belay Tolera 1* , Mulugeta Diro 2 and Derbew Belew 3

n vitro Aseptic Culture Establishment of Sugarcane ( Saccharum officinarum L.) Varieties Using Shoot Tip Explants

Belay Tolera 1* , Mulugeta Diro 2 and Derbew Belew 3
1 Ethiopian Sugar Corporation, Research and Training Division, Variety Development Directorate, Biotechnology Research Team, Wonji Research Center, P.O Box 15, Wonji, Ethiopia 2 Capacity Building for Scaling up of Evidence-based Best Practices in Agricultural Production in Ethiopia (CASCAPE) Project, Addis Ababa, Ethiopia 3 Jimma University, College of Agriculture and Veterinary Medicine, Jimma, Ethiopia
Abstract :

Two sugarcane varieties namely, ‘B41-227’ and ‘N14’ were tested for in vitro culture establishment response under five levels of BAP (0, 1, 2, 3 and 4 mgL -1 ) and kinetin (0, 0.5, 1, 1.5 and 2 mgL -1) in a completely randomized design with 2*5*5 factorial treatment combinations. The objective was to determine the optimum concentration and combination of BAP and kinetin for in vitro culture establishment of shoot tip explants. Among the different concentrations and combinations of BAP and kinetin tested, Murashige and Skoog (MS) medium supplemented with 3 mgL -1 BAP without kinetin for B41-227; and 3 mgL -1 BAP along with 1.5 mgL -lkinetin for N14 exhibited a better response than the other concentrations in percent initiated shoot tip explants, percent of dead or not responding cultures, number of shoots per explant and average shoot length of the sugarcane varieties. These mediaestablished 93.33% of shoot tip explants with 4.5 ± 0.11 shoots per explant and 3.15 ± 0.28 cm shoot length inB41-227; and initiated 76.67% shoot tip explants with 3.3 ± 00 shoots per explant and 4.58 ± 0.28 cm shoot length in N14 after 30 days of culture

Keywords :
Conventional propagation; In vitro shoot tip explant establishment; Sugarcane; BAP and kinetin

Date Deposited : 05 Apr 2016 16:22

Last Modified : 05 Apr 2016 16:22

Official URL: http://www.esciencecentral.org/journals/archive-advances-in-crop-science-and-technology-open-access.php

Volume 2, Number 3, August 2014 , ISSN ISSN: 2329-8863

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