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.: Home > Advances in Crop Science and Technology > 2014 > Volume 2 Number 3 > Belay Tolera 1* , Mulugeta Diro 2 and Derbew Belew 3

Effects of 6-Benzyl aminopurine and Kinetin on In Vitro Shoot Multiplication of Sugarcane ( Saccharum officinarum L.) Varieties

Belay Tolera 1* , Mulugeta Diro 2 and Derbew Belew 3
1 Ethiopian Sugar Corporation, Research and Training Division, Variety Development Directorate, Biotechnology Research Team, Wonji Research Center, Wonji, Ethiopia 2 Capacity Building for Scaling up of Evidence-based Best Practices in Agricultural Production in Ethiopia (CASCAPE) Project, Addis Ababa, Ethiopia 3 Jimma University, College of Agriculture and Veterinary Medicine, Jimma, Ethiopia
Abstract :

In vitro shoot multiplication of two commercial sugarcane varieties grown in Ethiopian sugar estates initiated using shoot  tip  explants  was  carried  out  with  the  objective  to  assess  the  multiplication  responses  of  the  sugarcane varieties (B41-227 and N14) under five levels of 6-benzylaminopurine (0, 0.5, 1, 1.5 and 2 mgL-1) and kinetin (0, 0.1, 0.25,  0.5,  and  1  mgL-1)  in  a  completely  randomized  design  with  5x5x2  factorial  treatment  combinations arrangements. Analysis of variance (ANOVA) showed that the interaction effects of 6-benzylaminopurine, kinetin and the sugarcane genotypes on number of shoots per explant, average shoot length and number of leaves per shoot  was  very  highly  significant  (P  <  0.0001).  Murashige  and  Skoog  (MS)  medium  fortified  with  1  mgL -1  6- benzylaminopurine  (BAP)  and  0.5  mgL-1  kinetin  for  B41-227;  and  2  mgL-1  6-benzylaminopurine  (BAP)  without kinetin for N14 were found to give optimum shoot proliferation results. On these medium, B41-227 gave 34 ± 1.54shoots per explant with 6.95 ± 0.01cm average shoot length and 12 ± 0.17 leaves per shoot while N14 produced 21 ± 0.58 shoots per explant with 5.63 ± 0.01 cm average shoot length and 5.4 ± 00 leaves per shoot after 30 days of culture  transfer  to  multiplication  medium.  Thus,  the  optimized  protocol  is  useful  for  rejuvenation,  rapid In  vitro propagation and production of large quantity quality sugarcane planting materials and hence help minimizes the major limitations hampering sugarcane production in the Ethiopian sugar Estates.

Keywords :
n vitro Shoot multiplication; Sugarcane; 6- benzylamminopurine (BAP); kinetin.

Date Deposited : 05 Apr 2016 16:28

Last Modified : 05 Apr 2016 16:28

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Volume 2, Number 3, August 2014 , ISSN ISSN: 2329-8863

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