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Advances in Crop Science and Technology

.: Home > Advances in Crop Science and Technology > 2014 > Volume 2 Number 3 > Suman Bakshi1*, Nayeem KA2 , Bhagwat SG1 , Shitre A1 and Das BK1

Characterization of GA3 Insensitive Reduced Height Mutant of Emmer Wheat Var. NP200 (Triticum Dicoccum)

Suman Bakshi1*, Nayeem KA2 , Bhagwat SG1 , Shitre A1 and Das BK1
1Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400 085, India 2 Indian Agricultural Research Institute, Regional Station, Wellington-643231, The Nilgiris, Tamil Nadu, India
Abstract :
A γ-ray induced semi-dwarf mutant was obtained in tall emmer wheat variety NP200. The mutant was insensitiveto externally applied gibberellin. An allele specific marker for dwarfing gene Rht-B1b was used to check the status of dwarfing gene in the mutant, semi dwarf and tall emmer and semi dwarf durum varieties. The primer showedamplification of Rht-B1b gene in semi dwarf durum and emmer varieties. The parent NP200 showed presence of wild type allele (Rht-B1a) with the primer pair BF-WR1. All semi dwarf emmer varieties showed a band of 237bp with primer pair BF-MR1showed presence of Rht-B1b. However, the mutant showed absence of amplification for both Rht-B1a and Rht-B1b alleles with respective primer pairs. The results indicated that the reduced height mutant carried a mutation different from the known allele Rht-B1b.
Keywords :
Triticum dicoccum; GA3 insensitivity; Reduced height mutant; Rht genes; Yielding ability; Allele specific primer

Date Deposited : 07 Apr 2016 14:16

Last Modified : 07 Apr 2016 14:16

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Volume 2, Number 3, August 2014 , ISSN ISSN: 2329-8863

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