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Advances in Crop Science and Technology

.: Home > Advances in Crop Science and Technology > 2014 > Volume 2 Number 2 > Miheretu Fufa1 * and Mulugeta Diro2

Microtuber Induction of Two Potato (Solanum tuberosum L.) Varieties

Miheretu Fufa1 * and Mulugeta Diro2
1Oromia Agricultural Research Institute, Adami Tullu Agricultural Research Center, Plant Biotechnology Team, P.O. Box 35, Zeway, Ethiopia 2Southern Agricultural Research Institute, Ethiopia
Abstract :
Two potato varieties namely, ‘Hunde’ and ‘Ararsa’ were tested for their microtuber induction under five levels of sucrose (40, 60, 80, 100 and 120 gl-1) in completely randomized design with 2×5 factorial combinations. The objective was to determine optimum concentration of sucrose for microtuber induction. In both varieties, among the five concentrations of sucrose, MS medium supplemented with 60 gl-1 sucrose exhibited a better response than the other concentrations in mean values of microtuber number, diameter, and weight and was found optimum. Accordingly, this medium produced, after 42.57 ± 0.58 days of culture, an average value of (1.97 ± 0.02) microtuber number, (3.60 ± 0.04 mm) microtuber diameter, and (0.08 ± 0.002 g) weight of microtuber in the variety Ararsa. On the other hand, it gave, after 35.67 ± 0.58 days of culture, mean value of (2.90 ± 0.031) microtuber number, (2.95 ± 0.01 mm) microtuber diameter, and (0.06 ± 0.001 g) weight of microtuber in the variety Hunde
Keywords :
In vitro tuberization; Microtuber; Potato; Sucrose

Date Deposited : 07 Apr 2016 14:27

Last Modified : 07 Apr 2016 14:27

Official URL: http://www.esciencecentral.org/journals/archive-advances-in-crop-science-and-technology-open-access.php

Volume 2, Number 2, April 2014 , ISSN ISSN: 2329-8863

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