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Advances in Crop Science and Technology

.: Home > Advances in Crop Science and Technology > 2014 > Volume 2 Number 2 > Aline Kadri1,2*, Lamis Chalak3, Ahmad El Bitar2, Nancy Nicolas2, Souad MrouƩ2 and Ghislaine Grenier De March4

In vitro Plant Regeneration System for Two Middle East Cultivars of Chickpea (Cicer arietinum L.)

Aline Kadri1,2*, Lamis Chalak3, Ahmad El Bitar2, Nancy Nicolas2, Souad MrouƩ2 and Ghislaine Grenier De March4
1Institut Polytechnique LaSalle Beauvais, France 2Lebanese Agricultural Research Institute (LARI), Lebanon 3Faculty of Agricultural Sciences, Lebanese University, Lebanon 4Champagne Ardenne Research & Innovation Agency (CARINNA), France
Abstract :
This study surveyed a rapid, efficient and reproducible protocol for in vitro shoot regeneration and rooting of two Middle East chickpea cultivars (Balila and Wady). Organogenic ability of various explants (decapitated embryo axes, fragments of embryo axes, fragments of embryo axes with basal part of cotyledons, entire and half of cotyledons) was tested on MS media containing different concentrations and nature of cytokinins (BAP and zeatin) or compound with cytokinin-like activity (TDZ). The highest level of shoot regeneration was achieved in Balila genotype using embryo axes fragments including the basal part of cotyledon on medium with 0.25 mg/L BAP. Higher BAP concentrations than 0.25 mg/L for Balila and 0.5 mg/L for Wady caused a decrease of shoot regeneration. On MS media supplemented with zeatin (1, 3 and 5 mg/L) and thidiazuron (1, 3 and 5 mg/L), the highest level of shoot differentiation was obtained from calli derived from fragments of embryo axes. Organogenic ability of calli from Wady genotype was enhanced by increasing zeatin and TDZ concentrations, up to 5 mg/L. Conversely, a progressive decrease of shoot frequency for Balila and root development for both genotypes, were observed with increasing concentrations of BAP, zeatin and TDZ. 
Keywords :
Chickpea, Middle East cultivars, Organogenic ability, Cytokinins, Explants types

Date Deposited : 07 Apr 2016 14:40

Last Modified : 07 Apr 2016 14:40

Official URL: http://www.esciencecentral.org/journals/archive-advances-in-crop-science-and-technology-open-access.php

Volume 2, Number 2, April 2014 , ISSN ISSN: 2329-8863

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