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.: Home > Nature and Science > 2012 > Volume 10 Number 5 > Adebare Johnson Adeleke, Sunday Ayodele Odunfa, Afolake Olanbiwonninu, Mojisola Christiana Owoseni

Production of Cellulase and Pectinase from Orange Peels by Fungi

Adebare Johnson Adeleke, Sunday Ayodele Odunfa, Afolake Olanbiwonninu, Mojisola Christiana Owoseni
Department of Microbiology, University of Ibadan, Nigeria. lekejohnson2222@yahoo.ca
Abstract :

The aim of the work was to evaluate the potentials of fungi to produce pectinase and cellulase using orange peels as substrate. Fungi were isolated and identified from soil and decomposing orange peels. Fungal isolates were screened in modified Czapek-Dox media with carboxymethylcellulose and citrus pectin as the only carbon source for endoglucanase and polygalacturonase production respectively. Out of a total thirteen isolates, three highest producers of the enzymes were selected and identified as Penicillium atrovenetumAspergillus flavus and Aspergillus oryzae. The three isolates were further used to ferment orange peels in a solid state fermentation. The effects of incubation time, pH, temperature and nitrogen sources on the level of production of the enzymes were investigated. The three isolates produced polygalacturonase optimally on the 5th day while endoglucanase was produced optimally on the 7th day. Highest production of polygalacturonase and endoglucanase by Penicillium atrovenetum was observed at pH 5, 40oC and at 0.2% ammonium persulfate. Maximum production of polygalacturonase and endoglucanase by Aspergillus flavus was observed at pH 5.5, 40oC and 0.25% ammonium persulfate while Aspergillus oryzae produced the two enzymes maximally at pH 5.5, 35oC and 0.2% ammonium persulfate.

Keywords :
endoglucanase; polygalacturonase; fungi; orange peels

Date Deposited : 16 May 2016 20:46

Last Modified : 16 May 2016 20:46

Official URL: http://www.sciencepub.net/nature/

Volume 10, Number 5, - 2012 , ISSN 1545-0740

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