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.: Home > Animal Science Papers and Reports > 2010 > Volume 28 Number 1 > Agnieszka Iwanowska1, Ewa Iwańska1, Bożena Grześ1, Beata Mikołajczak1, Edward Pospiech1,2,**, Stanisław Rosochacki3,4, Edyta Juszczuk-Kubiak3, Andrzej Łyczyński5

Changes in proteins and tenderness of meat from young bulls of four breeds at three ages over 10 day

Agnieszka Iwanowska1, Ewa Iwańska1, Bożena Grześ1, Beata Mikołajczak1, Edward Pospiech1,2,**, Stanisław Rosochacki3,4, Edyta Juszczuk-Kubiak3, Andrzej Łyczyński5
1 Institute of Meat Technology, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, Poland, 2 Meat and Fat Research Institute in Poznan, Głogowska 239, 60-111 Poznań, Poland, 3 Institute of Genetics and Animal Breeding, Jastrzębiec, 05-552 Wólka Kosowska, Poland, 4 Chair of Sanitary Biology and Biotechnology, Białystok Technical University, Wiejska 45e, 15-351 Białystok, Poland, 5 Department of Animal Origin Materials, Poznań University of Life Sciences, Wołyńska 33, 60-637 Poznań, Poland
Abstract :

The study aimed at analysing the changes taking place in the muscle tissue of cattle during meat cold storage in relation to animal genotype (breed) and age. Investigations were conducted on the thoracic and lumbar part of the longissimus dorsi (LD) muscle of Polish Holstein-Friesian (PHF) Black-and-White variety, Polish Red (PR), Hereford (H) and Limousine (L) bulls slaughtered at the age of 6, 9 and 12 months. Muscle analyses were carried out 45 min and 48, 96 and 240 h postslaughter, separating  proteins with the assistance of SDS-PAGE (electrophoresis in polyacrylamide gel using SDS). To identify titin, desmin and troponin T (Tn-T), their antibodies and western blotting were employed. The breed occurred to be the key factor affecting proteins’ changes in the muscle tissue. The process of protein degradation in PHF was similar to that found in H while in PR – to that occurring in L bulls, despite the genotype differences between them. The greatest differences in protein changes were found between the meat obtained from bulls at the age of 6 vs. 12 months. During meat cold storage, day 10 turned out to be critical with regard to the degradation of almost all proteins. The drop of the content of titin, desmin and Tn-T was observed, simultaneously with the increase in their degradation products. The highest myofibrillar protein degradation observed on day 10 of cold storage proves these changes.

Keywords :
beef, tenderness, myofibrillar proteins, proteolysis, SDS-PAGE, immunoblotting

Date Deposited : 10 Jan 2011 17:06

Last Modified : 11 Jan 2011 11:22

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34&o=2832

Volume 28, Number 1, - 2010

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