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.: Home > Nature and Science > 2012 > Volume 10 Number 9 > Hazim J. Al-Daraji

Red wine as a diluent supplement for counteract the deleterious effects of lipid peroxidation during liquid storage of aged roosters semen

Hazim J. Al-Daraji
Department of Animal Production, College of Agriculture, University of Baghdad, Baghdad, Iraq
Abstract :

This study is an attempt to enhance the resistance of roosters' semen to peroxidative damage during in vitrostorage by supplementing the semen diluent of cockerels with the powerful antioxidant red wine. Cockerels at 65 weeks of age were randomly distributed into six treatment groups of 10 birds each. Semen was collected twice a week from all cockerels during the whole period of experiment (12 consecutive weeks). Fresh semen served as a control (T1); Treatments were semen extended with Al – Daraji 2 diluent (AD2D) alone (T2); extended semen supplemented with 4, 8, 12 or 16 ml of red wine / 100 ml of AD2D (T3, T4, T5 or T6, respectively). Semen samples were then stored at 4 – 6ºC for 24 h, 48 h or 72 h. Results revealed that the addition of red wine into semen diluent (T3, T4, T5 and T6) resulted in significant (p < 0.01) improvement in mass activity, individual motility, viability and percentages of normal spermatozoa and intact acrosomes compared to T1 and T2 groups. Moreover, T6 (16 ml red wine / 100 ml of diluent) recorded the best results in relation to these five traits followed by the results of T5, T4 and T3, respectively. In conclusion, the supplementation of semen diluent composition with red wine significantly ameliorates quality of aged roosters semen that in vitro stored for up to 72 h. However, the beneficial effect of red wine found in the present study may be due to enhance sperm resistance to lipid peroxidation that naturally occurred during in vitro storage of avian semen.

Keywords :
red wine, diluent, lipid peroxidation, liquid storage, semen, aged roosters.

Date Deposited : 18 May 2016 19:45

Last Modified : 18 May 2016 19:45

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Volume 10, Number 9, - 2012 , ISSN 1545-0740

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