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.: Home > Nature and Science > 2012 > Volume 10 Number 9 > Rasha A. Nasr, Makram F. Attalah

Molecular Epidemiology of Nosocomial Acinetobacter baumannii Isolates

Rasha A. Nasr, Makram F. Attalah
Department of Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Rnasr2@hotmail.com
Abstract :

 In view of the high rate and rapid spread of multidrug-resistant (MDR) Acinetobacter baumannii causing nosocomial infections especially in ICUs, this study was conducted to elucidate the antimicrobial susceptibility and the molecular epidemiology of the A. baumannii isolated from nosocomial infections in 3 ICUs in the El-Zaitoun Specialized Hospital, Cairo. During a 7-month study period, a total of 20 A. baumannii were isolated from nosocomial infections and environmental sources in the 3 ICUs. Susceptibility of the isolates to different antimicrobials was determined by the disk diffusion method. Molecular typing of all isolates was performed using the random amplified polymorphic DNA (RAPD)-PCR assay. A. baumannii were most frequently isolated from endotracheal aspirates (14), 4 strains from post-operative wound infection and 2 from environmental samples. All isolates were MDR and were totally resistant to imipenem, ampicillin/sulbactam, ceftazidime, ciprofloxacin, piperacillin/tazobactam and ceftriaxone. A high resistance rate was observed to amikacin and trimethoprim/sulfamethoxazole (90% each) gentamicin (85%) and doxycycline (75%). Molecular typing revealed circulation of 8 RAPD-fingerprints, of which fingerprint A accounted for 50% of A. baumannii strains including the 2 environmental isolates. Fingerprint B comprised 20% while the other isolates showed different RAPD-fingerprints. In conclusion, there was an increase in the rate of MDR A. baumannii in the ICUs which necessitates the implementation of an appropriate antibiotic policy. The intrahospital spread of especially one RAPD fingerprint of A. baumannii and its isolation from environmental sources emphasize the need of strict adherence to infection control measures in hospitals as well as the value of molecular typing to investigate spread of infection

Keywords :
Acinetobacter baumannii, intensive care units, multidrug-resistant, RAPD-PCR

Date Deposited : 18 May 2016 19:47

Last Modified : 18 May 2016 19:47

Official URL: http://www.sciencepub.net/nature/

Volume 10, Number 9, - 2012 , ISSN 1545-0740

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