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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2010 > Volume 9 Number 1 > G.S. Niture1, A.G. Karpe2, Minakshi Prasad3, A.V. Bhonsle4 and S.V. Patil1

Detection of Group D Avian Rotaviruses among Layer Poultry from Western India

G.S. Niture1, A.G. Karpe2, Minakshi Prasad3, A.V. Bhonsle4 and S.V. Patil1
1Department of Animal Biotechnology, College of Veterinary and Animal Sciences, Parbhani 431 402, Maharashtra State, India 2KNP College of Veterinary Sciences, Shirwal, India 3Department of Animal Biotechnology, CCS HAU, Hisar, India 4Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Udgir
Abstract :

Rotavirus is a major cause of gastroenteritis in infants and children worldwide and also in wide
variety of mammalian and avian species. In the present study, 4 (7.84%) out of the 54 poultry faecal samples tested, were found positive by RNA-PAGE. The samples were of 60-65 weeks age layer poultry birds suffering from diarrhoea. All the PAGE positive samples resolved in to 11 segments with a migration pattern of 5:2:2:2 characteristic of group D avian rotavirus. All the positive samples were of short electropherotype in which segments 10th and 11th migrated very closely. Avian rotavirus samples subjected to VP4 and VP7 gene based RT-PCR did not amplified respective products. RNA-PAGE positive samples were further subjected to VP6 gene based RT-PCR amplification using specific primers. All the four positive samples yielded a specific product of 493 bp. The study reports first ever detection of Group D avian rotavirus in Western India.

Keywords :
Avian rotavirus, group D, RNA PAGE, RT-PCR, VP6

Date Deposited : 25 Jun 2011 10:05

Last Modified : 25 Jun 2011 10:05

Official URL: http://www.pjbs.org/ijps/ijps.htm

Volume 9, Number 1, - 2010 , ISSN 1682-8356

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