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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2010 > Volume 9 Number 1 > P. Gong, Y.Z. Gong, Y.P. Yang, Y. Yang, Y.P. Feng, S.J. Li and X.L. Peng

Inducible Mis- and Over-Expression of PKCIW Regulates the Expression of AMH and P450arom in Chicken Embryo Fibroblast

P. Gong, Y.Z. Gong, Y.P. Yang, Y. Yang, Y.P. Feng, S.J. Li and X.L. Peng
College of Animal Sciences and Technology, Huazhong Agricultural University,430070, Wuhan, Hubei Province, P.R. China
Abstract :

Sex in birds is determined genetically as in mammals and a Z-linked gene DMRT1 is required for
testis development. But it remains possible that the W chromosome contains a similarly critical factor for
female development. The W-linked gene PKCIW was put forward as the seductive candidate for avian sex
determination and/or sex differentiation. Mis-expression and over-expression of PKCIW were performed
respectively in male and female chicken embryo fibroblast with tet-on inducible expression system in this
study. The results of RT-PCR showed that with the PKCIW recombinant vector introducing, PKCIW mRNA
expression was significantly improved in female chicken embryo fibroblast and was close to that of the
female controls and in male chicken embryo fibroblast, in which normally there was no expression. Both misand
over-expression of PKCIW led to up-regulated expression of P450arom and down-regulated expression
of AMH, while large increase of P450arom in female and large decrease of AMH in male appeared. With misand
over-expression of PKCIW, there was no significant change of FOXL2, SOX9, DMRT1 and ATP5A1W
mRNA expression. These results showed the possible regulatory effect of PKCIW on AMH and P450arom
in transcription level.

Keywords :
Chicken, sex determination, PKCIW, mis-expression, over-expression, tet-on

Date Deposited : 25 Jun 2011 10:15

Last Modified : 25 Jun 2011 10:15

Official URL: http://www.pjbs.org/ijps/ijps.htm

Volume 9, Number 1, - 2010 , ISSN 1682-8356

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