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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2016 > Volume 65 Number 1 > Fangui Min1,2), Jinchun Pan1,2), Ruike Wu1,2), Meiling Chen1,2), Huiwen Kuang1,2), and Weibo Zhao1,2)

Profiling serum antibodies to Mycobacterium tuberculosis proteins in rhesus monkeys with nontuberculous Mycobacteria

Fangui Min1,2), Jinchun Pan1,2), Ruike Wu1,2), Meiling Chen1,2), Huiwen Kuang1,2), and Weibo Zhao1,2)
1)Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, P.R. China 2)Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663, P.R. China
Abstract :

Recent evidence indicates that the prevalence of diseases caused by nontuberculous mycobacteria (NTM) has been increasing in both human and animals. In this study, antibody profiles of NTM in rhesus monkeys (Macaca mulatta) were determined and compared with those of monkeys infected with Mycobacterium tuberculosis complex (MTBC). Antibodies against 10 M. tuberculosis proteins, purified protein derivative (PPD), and mammalian old tuberculin (MOT) were detected in 14 monkeys naturally infected with NTM by indirect ELISA. Sera from 10 monkeys infected with MTBC and 10 healthy monkeys were set as controls. All antigens showed high serological reactivities to MTBC infections and low reactivities in healthy monkeys. NTM infections showed strong antibody responses to MOT and PPD; moderate antibody responses to 16kDa, U1, MPT64L, 14kDa, and TB16.3; and low antibody responses to 38kDa, Ag85b, CFP10, ESAT-6, and CFP10-ESAT-6. According to the criteria of MTBC, only CFP10, ESAT-6, and CFP10-ESAT-6 showed negative antibody responses in all NTM infections. Taken together, these results suggest that positive results of a PPD/MOT-based ELISA in combination with results of antibodies to M. tuberculosis-specific antigens, such as CFP10 and ESAT-6, could discriminate NTM and MTBC infections. Two positive results indicate an MTBC infection, and a negative result for an M. tuberculosis-specific antigen may preliminarily predict an NTM infection.

Keywords :
antibody, M. tuberculosis proteins, nontuberculous mycobacteria, rhesus monkeys

Date Deposited : 24 Jun 2016 18:52

Last Modified : 24 Jun 2016 18:52

Official URL: http://

Volume 65, Number 1, - 2016 , ISSN 1341-1357

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