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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2016 > Volume 12 Number 6 > Ningbo Wang, Mengjie Liu, Lihua Guo, Xiufen Yang, Dewen Qiu 

A Novel Protein Elicitor (PeBA1) from Bacillus amyloliquefaciens NC6 Induces Systemic Resistance in Tobacco

Ningbo Wang, Mengjie Liu, Lihua Guo, Xiufen Yang, Dewen Qiu 
The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant protection, Chinese Academy of Agricultural sciences, No. 12 Zhongguancun South Street, Beijing 100081, China  Corresponding author: Dewen Qiu. E-mail address: qiudewen@caas.cn; Telephone/Fax: +86 10 82105924
Abstract :

Here we reported a novel protein elicitor from Bacillus amyloliquefaciens NC6 induced systemic resistance (ISR) in tobacco. The purification was executed by ion-exchange chromatography, native-page extraction and HPLC, and the amino acid sequence was identified by mass spectrometry. This recombinant elicitor protein, expressed in Escherichia coli by an E1 expression vector, had good thermal stability, and the elicitor caused a clearly defined hypersensitive response (HR) necrosis in tobacco leaves. It could also trigger early defence events, including generation of reactive oxygen species (H2O2 and O2 - ) and phenolic-compound accumulation. Quantitative real-time PCR (Q-RT-PCR) results indicated that several plant defence genes, including the salicylic acid (SA)-responsive PR1a, PR1b, PR5, and phenylalanine ammonia lyase (PAL), as well as the jasmonic acid (JA)-responsive PDF1.2 and CORONATINE INSENSITIVE 1 (COI1), were all up-regulated. Moreover, infiltration conferred systemic resistance against a broad spectrum of pathogens, including Tobacco mosaic virus (TMV) and the fungal pathogen Botrytis cinerea.

Keywords :
protein elicitor; induced systemic resistance; ROS; Hypersensitive response

Date Deposited : 04 Nov 2016 22:15

Last Modified : 04 Nov 2016 22:15

Official URL: http://www.ijbs.com/v12i6

Volume 12, Number 6, April 2016

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