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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2016 > Volume 12 Number 7 > Sung Jin Cho1*, Jihoo Lee2*, Hyun Jae Lee1, Hyun-Young Jo3, Mangalam Sinniah4, Hak-Yong Kim2,, Chom-Kyu Chong5,, Hyun-Ok Song6,

A Novel Malaria Pf/Pv Ab Rapid Diagnostic Test Using a Differential Diagnostic Marker Identified by Network Biology

Sung Jin Cho1*, Jihoo Lee2*, Hyun Jae Lee1, Hyun-Young Jo3, Mangalam Sinniah4, Hak-Yong Kim2,, Chom-Kyu Chong5,, Hyun-Ok Song6,
1. Department of Bioinformatics, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea 2. Department of Biochemistry, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea 3. Laboratory Medicine, Chungbuk National University Hospital, Cheongju, Chungbuk, Republic of Korea 4. Hospital Kuala Lumpur, Jalan Pahang, Malaysia 5. GenBody Inc., Dankook Biotech Business IC, Cheonan, Chungnam, Republic of Korea 6. Department of Infection Biology, Wonkwang University School of Medicine, Iksan, Jeonbuk, Republic of Korea * Sung Jin Cho and Jihoo Lee contributed equally to this work  Corresponding authors: Hak-Yong Kim: Tel +82-43-261-2307, E-mail: hykim@chungbuk.ac.kr. Chom-Kyu Chong: Tel +82-41-523-8990, Fax +82-41-523-8991, E-mail: ckchong@genbody.co.kr. Hyun-Ok Song: Tel +82-63-850-6972, E-mail: hosong@wku.ac.kr.
Abstract :

Rapid diagnostic tests (RDTs) can detect anti-malaria antibodies in human blood. As they can detect parasite infection at the low parasite density, they are useful in endemic areas where light infection and/or re-infection of parasites are common. Thus, malaria antibody tests can be used for screening bloods in blood banks to prevent transfusion-transmitted malaria (TTM), an emerging problem in malaria endemic areas. However, only a few malaria antibody tests are available in the microwell-based assay format and these are not suitable for field application. A novel malaria antibody (Ab)-based RDT using a differential diagnostic marker for falciparum and vivax malaria was developed as a suitable high-throughput assay that is sensitive and practical for blood screening. The marker, merozoite surface protein 1 (MSP1) was discovered by generation of a Plasmodium-specific network and the hierarchical organization of modularity in the network. Clinical evaluation revealed that the novel Malaria Pf/Pv Ab RDT shows improved sensitivity (98%) and specificity (99.7%) compared with the performance of a commercial kit, SD BioLine Malaria P.f/P.v (95.1% sensitivity and 99.1% specificity). The novel Malaria Pf/Pv Ab RDT has potential for use as a cost-effective blood-screening tool for malaria and in turn, reduces TTM risk in endemic areas

Keywords :
Malaria antibody test, MSP1, Transfusion-transmitted malaria, Blood screening

Date Deposited : 04 Nov 2016 22:30

Last Modified : 04 Nov 2016 22:30

Official URL: http://www.ijbs.com/v12i7

Volume 12, Number 7, May 2016

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