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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2010 > Volume 9 Number 2 > X.X. Li1,3, L.X. Han2 and J.L. Han3,4

No Specific Primer Can Independently Amplify the Complete Exon 2 of Chicken BLB1 or BLB2 Genes

X.X. Li1,3, L.X. Han2 and J.L. Han3,4
1College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China 2Laboratory Animal Center of Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Harbin 150001, China 3Key Laboratory of Farm Animal Genetic Resources and Utilization, Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China 4CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China
Abstract :

Chicken BLB1 and BLB2 genes are duplicated within MHC-B region that plays a crucial role in
disease resistance or susceptibility. To investigate the genetic polymorphism in chicken BLB genes, we
analyzed the complete genomic DNA sequences of BLB1 and BLB2 genes from 14 published MHC-B
haplotypes (59 kb). Two pairs of primers were chosen or designed to amplify the exon 2 fragments of both
genes from six known MHC-B haplotypes. The PCR products were directly sequenced for a preliminary
identification of specific variations that were further validated using cloning approach. We found that the
specificity of the primers becomes ambiguous to nearly all of the MHC-B haplotypes. Therefore it is
impossible to design specific primer according to the complete exon and intron sequences for an
independent amplification of complete exon 2 of the BLB1 or BLB2

Keywords :
Chicken, MHC, BLB1, BLB2, exon 2, polymorphism

Date Deposited : 25 Jun 2011 10:39

Last Modified : 25 Jun 2011 10:39

Official URL: http://www.pjbs.org/ijps/ijps.htm

Volume 9, Number 2, - 2010 , ISSN 1682-8356

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