International Journal Of Bilogical Sciences
.: Home > International Journal Of Bilogical Sciences > 2016 > Volume 12 Number 10 > Yingxue Yu1,3*, Xuehua Sun2*, Jinyang Gu1*, Chang Yu1,3, Yankai Wen1,3, Yueqiu Gao2, Qiang Xia1, and Xiaoni Kong1
Deficiency of DJ-1 Ameliorates Liver Fibrosis through Inhibition of Hepatic ROS Production and Inflammation
Yingxue Yu1,3*, Xuehua Sun2*, Jinyang Gu1*, Chang Yu1,3, Yankai Wen1,3, Yueqiu Gao2, Qiang Xia1, and Xiaoni Kong1
1. Department of Liver Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. 2. Department of liver diseases, Shuguang Hospital affiliated to Shanghai University of Chinese Traditional Medicine, Shanghai, China. 3. School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China. *These authors contributed equally to this work. Corresponding authors: Xiaoni Kong, PhD., Department of Liver Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pujian Road, Shanghai, China. 200127. Telephone: +862168383283; E-mail: email@example.com; firstname.lastname@example.org or Qiang Xia, MD., E-mail: email@example.com or Yueqiu Gao, MD., Email: firstname.lastname@example.org.
Liver fibrosis is a global health problem and previous studies have demonstrated that reactive oxygen species (ROS) play important roles in fibrogenesis. Parkinson disease (autosomal recessive, early onset) 7 (Park7) also called DJ-1 has an essential role in modulating cellular ROS levels. DJ-1 therefore may play functions in liver fibrogenesis and modulation of DJ-1 may be a promising therapeutic approach. Here, wild-type (WT) and DJ-1 knockout (DJ-1 KO) mice were administrated with carbon tetrachloride (CCl4) to induce liver fibrosis or acute liver injury. Results showed that DJ-1 depletion significantly blunted liver fibrosis, accompanied by marked reductions in liver injury and ROS production. In the acute CCl4 model, deficiency of DJ-1 showed hepatic protective functions as evidenced by decreased hepatic damage, reduced ROS levels, diminished hepatic inflammation and hepatocyte proliferation compared to WT mice. In vitro hepatic stellate cells (HSCs) activation assays indicated that DJ-1 has no direct effect on the activation of HSCs in the context of with or without TGFβ treatment. Thus our present study demonstrates that in CCl4-induced liver fibrosis, DJ-1 deficiency attenuates mice fibrosis by inhibiting ROS production and liver injury, and further indirectly affecting the activation of HSCs. These results are in line with previous studies that ROS promote HSC activation and fibrosis development, and suggest the therapeutic value of DJ-1 in treatment of liver fibrosis.
DJ-1; liver fibrosis; liver injury; ROS; inflammation; lipid peroxidation.
Date Deposited : 07 Nov 2016 20:39
Official URL: http://www.ijbs.com/v12i10
Last Modified : 07 Nov 2016 20:39
Volume 12, Number 10, September 2016
Full Text Original