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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2016 > Volume 12 Number 10 > Fan Lin*, Liang Dong*, Weiming Wang, Yuchen Liu, Weiren Huang, Zhiming Cai

An Efficient Light-Inducible P53 Expression System for Inhibiting Proliferation of Bladder Cancer Cell

Fan Lin*, Liang Dong*, Weiming Wang, Yuchen Liu, Weiren Huang, Zhiming Cai
State Engineering Laboratory of Medical Key Technologies Application of Synthetic Biology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518039, PR China. *These authors have contributed equally to this work.  Corresponding authors: Dr. Weiren Huang Email: Or Prof. Zhiming Cai Email:
Abstract :

Optogenetic gene expression systems enable spatial-temporal modulation of gene transcription and cell behavior. Although applications in biomedicine are emerging, the utility of optogenetic gene switches remains elusive in cancer research due to the relative low gene activation efficiency. Here, we present an optimized CRISPR-Cas9-based light-inducible gene expression device that controls gene transcription in a dose-dependent manner. To prove the potential utility of this device, P53 was tested as a functional target in the bladder cancer cell models. It was illustrated that the light-induced P53 inhibited proliferation of 5637 and UMUC-3 cell effectively. The “light-on” gene expression system may demonstrate a novel therapeutic strategy for bladder cancer intervention.

Keywords :
light-inducible gene expression; CRISPR-Cas9; gene switch; bladder cancer.

Date Deposited : 07 Nov 2016 20:43

Last Modified : 07 Nov 2016 20:43

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Volume 12, Number 10, October 2016

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