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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2010 > Volume 9 Number 3 > S.K. Singh1, S. Mehra1, S.K. Shukla1, Vinay Kumar1, A. Tiwari1, M. Mehra1,

Nucleotide Sequence Variation in MHC Class I Region in Guinea Fowl

S.K. Singh1, S. Mehra1, S.K. Shukla1, Vinay Kumar1, A. Tiwari1, M. Mehra1,
Giriraj Goyal1, Jose Mathew2 and Deepak Sharma1 1Genome Mapping Laboratory, Central Avian Research Institute, Izatnagar 243 122, India 2Department of Biotechnology, Bundelkhand University, Jhansi, India
Abstract :

The MHC class I gene was amplified, cloned and sequenced in guinea fowl using the primers
specific to BF2 gene in chicken. The nucleotide sequence of 571 bp partial CDS of BF2 gene includes 32
nucleotides of signal peptide (exon 1), complete " domain (270 nucleotides) and 269 nucleotides of " 1 2
domain. For " and " domain no sequence variation was observed within guinea fowl sequences, however, 1 high variability was observed within the other poultry species (15.93-28.03%) except chicken (7.95-9.16%). Between the guinea fowl and other poultry species, the " domain showed high nucleotide variability (29.26- 1 43.70%). Among poultry species, guinea fowl showed least variability with chicken and maximum with duck. Among the substitutions, majorities were of non-synonymous (76.27%) with a ration of 1:3 between synonymous to non-synonymous substitutions. Guinea fowl showed lower genetic distances (Kimura 2- parameter) with chicken and quail (0.211-0.215), while with duck and goose, it showed higher genetic distances (0.343-0.350). Phylogenetic tree, based these genetic distances revealed two major clusters, comprising of guinea fowl, quail and chicken in one with guinea fowl as separate branch, while duck and goose in other.

Keywords :
MHC I, guinea fowl, phylogeny, nucleotide variability

Date Deposited : 25 Jun 2011 11:28

Last Modified : 25 Jun 2011 11:28

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Volume 9, Number 3, - 2010 , ISSN 1682-8356

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