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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2010 > Volume 9 Number 4 > Q.R. Hou, J.Y. Wang, H.H. Wang, Y. Li, G.X. Zhang, Y. Wei and Hassan

Analysis of Polymorphisms in Exons of the LYZ Gene and Effect on Growth Traits of Jinghai Yellow Chicken

Q.R. Hou, J.Y. Wang, H.H. Wang, Y. Li, G.X. Zhang, Y. Wei and Hassan
College of Animal Science and Technology, Yangzhou University, Jiangsu Province, 225009, China
Abstract :

Lysozyme plays important role in vertebrates and invertebrates for its unique protein structure and
enzyme activity. In the present study, the potential physiological function of the chicken lysozyme gene (LYZ)
was studied by SNP. 329 J+ line Jinghai Yellow chicken were involved in this experiment. To determine gene
mutations, DNA analysis of the LYZ gene, including Single Strand Confirmation Polymorphism (SSCP) and
direct DNA sequencing, was performed. Chicken Body Weight (BW) were analyzed by GLM of SAS software.
DNA sequencing demonstrated a G111A transition of exon 1, a T1426C transition and a C1492T transition
of exon 2, all of them were silent mutations that caused no alteration in amino acid sequence. Statistical
analysis indicated that, AA and CC genotype related with high BW, TT and CN genotype contributed to low
BW. Therefore, results suggested that there was a possibility of the LYZ gene SNPs acting as a genetic
marker for growth traits of Jinghai Yellow chicken.

Keywords :
Chicken, genotype, growth traits, LYZ gene, polymorphism, SSCP

Date Deposited : 25 Jun 2011 13:20

Last Modified : 25 Jun 2011 13:20

Official URL: http://www.pjbs.org/ijps/ijps.htm

Volume 9, Number 4, - 2010 , ISSN 1682-8356

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