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.: Home > Animal Science Papers and Reports > 2010 > Volume 28 Number 4 > Amrit Kaur Bansal*, Gurmail Singh Bilaspuri

Effect of vitamin E and Mn2+ on the thiol status of cattle bull spermatozoa under induced oxidative stress

Amrit Kaur Bansal*, Gurmail Singh Bilaspuri
Department of Animal Reproduction, Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, India
Abstract :

Ferrous ascorbate – FeAA (FeSO4 + ascorbic acid) – has been used in the past to induce oxidative stress. In this study, the effects of various doses of vitamin E (1 mM, 2 mM, 2.5 mM) and Mn2+ (60 μM, 100 μM, 200 μM) on total thiol (TSH), glutathione reduced (GSH), glutathione oxidized (GSSG), and redox ratio (GSH/GSSG) were determined in the local crossbred cattle bull spermatozoa. Fresh semen was suspended in 2.9% sodium citrate and the suspension was divided into eight equal fractions. All fractions, i.e. control (containing 2.9% sodium citrate + spermatozoa) and experimental, i.e. treated / untreated with ferrous ascorbate (150 μM FeSO4 : 750 μM ascorbic acid), supplemented / unsupplemented with three doses of vitamin E (1 mM, 2 mM, 2.5 mM) / Mn2+ (60 μM, 100 μM, 200 μM) were incubated for 2 h at 37°C. These fractions were used for thiol components assessment. By inducing oxidative stress, FeAA decreased the TSH and GSH levels and GSH / GSSG ratio, but increased the GSSG level. All doses of vitamin E and Mn2+ improved the TSH and GSH level and GSH / GSSG ratio, but decreased GSSG level under normal and oxidative stress conditions. It is concluded that oxidative stress treatment (FeAA) decreased (P≤0.05) TSH and GSH contents but maintained GSSG concentration and GSH/GSSG ratio unchanged (P≥0.05). However, supplementation with vitamin E increased (P≤0.05) both the GSH content and GSH/GSSG ratio, but Mn2+ increased (P≤0.05) the GSH content only.

Keywords :
cattle, Mn2+, spermatozoa, thiol, vitamin E

Date Deposited : 11 Jan 2011 13:52

Last Modified : 11 Jan 2011 13:52

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34&o=3018

Volume 28, Number 4, - 2010

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