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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2010 > Volume 9 Number 11 > S. Shivaramaiah , J.R. Barta , S.L. Layton 1 2 1, C. Lester1, Y.M. Kwon1, L.R. Berghman3, B.M. Hargis1 and G. Tellez1

Development and Evaluation of an aroA / htrA Salmonella enteritidis Vector Expressing Eimeria maxima TRAP Family Protein EmTFP250 with CD 154 (CD 40L) as Candidate Vaccines against Coccidiosis in Broilers

S. Shivaramaiah , J.R. Barta , S.L. Layton 1 2 1, C. Lester1, Y.M. Kwon1, L.R. Berghman3, B.M. Hargis1 and G. Tellez1
1Department of Poultry Science, University of Arkansas, Fayetteville, AR, USA 2Department of Pathobiology, University of Guelph, Ontario, Canada 3Department of Poultry Science, Texas A&M University, College Station, TX, USA
Abstract :

Coccidiosis is caused by parasites of the genus Eimeria, belonging to phylum Apicomplexa. EmTFP250 is a high molecular mass, asexual stage antigen from Eimeria maxima (EM) strongly associated with maternal immunity in newly hatched chickens. Cloning and sequence analysis predict the antigen to be a novel member of the Thrombospondin-Related Adhesive Protein (TRAP) family. Three novel attenuated Salmonella enteritidis strains ()SE) expressing TRAP oligopeptides in association with a potential immuneenhancing CD 154 sequence, on the outer membrane protein lamB, were developed. Broiler chicks were grouped based on treatment and 108 cfu/chick of vectors expressing one of three sequences, or vehicle alone, was orally administered to each group. At 21 d of age, all groups were challenged with 104 sporulated oocysts/chick orally. Mortality at 5d post-challenge was markedly different (p<0.05) in chickens vaccinated with TRAP Upstream (US). To further evaluate the efficacy of TRAP US as a potential vaccine candidate, a similar study was conducted. Broilers were orally vaccinated with 108 cfu/chick vehicle with TRAP US and CD 154 or sham vaccinated with saline. Coccidia challenge was performed with 105 sporulated oocysts/chick at 22 d of age. Immunized chickens showed remarkable improvement in weight gain (p<0.05) and had reduced mortality (p = 0.055) when compared to non-immunized controls. These two studies underscore the potential of EmTFP250 as a potential candidate for a recombinant vaccine targeting coccidiosis in chickens.

Keywords :
Eimeria, Salmonella, coccidiosis, apicomplexa, TRAP, vaccination

Date Deposited : 28 Jun 2011 12:58

Last Modified : 28 Jun 2011 12:58

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Volume 9, Number 11, - 2010 , ISSN 1682-8356

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