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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2010 > Volume 9 Number 11 > Irungbam Karuna Devi , Y. Geeta Devi , Kh. Victoria Chanu 1 2 3, M. Ayub Ali3, Jully Gogoi Tewari4, M. Prava3, David Malsawmkima5 and Gautam Patra*

Transmission Electron Microscopy (TEM) Study of the Oocyst of Eimeria tenella

Irungbam Karuna Devi , Y. Geeta Devi , Kh. Victoria Chanu 1 2 3, M. Ayub Ali3, Jully Gogoi Tewari4, M. Prava3, David Malsawmkima5 and Gautam Patra*
*Department of Veterinary Parasitology, 3Department of Veterinary Physiology and Biochemistry, 4Department of Veterinary Public Health, 5Department of Veterinary Microbiology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India 1Department of Veterinary Physiology and Biochemistry, Veterinary College, Bidar, Nandinagar, KVAFSU, Karnataka, India 2Department of Pathology, All India Institute of Medical Sciences, Ansarinagar, New Delhi, India
Abstract :

The present work describes isolation, cell wall preparation, morphological as well as molecular detection of oocyst of Eimeria tenella. Based on transmission electron microscopy, the oocyst of this species showed the outer and inner wall without veil forming bodies; cross section of sporozoites with different
organelles and macrogamete structure at ultrastructural level. Polymerase Chain Reaction (PCR) based on Internal Transcribed Spacer 1 (ITSI) regions of ribosomal DNA (rDNA) demonstrated 270 bp specific for E. tenella. Results of this study provide the basis for further ultrastructural studies of the organism. The present study also suggested that morphological characteristics along with molecular identification are prerequisite for validation and identification of Eimeria species.

Keywords :
Oocyst, Eimeria tenella, organism

Date Deposited : 28 Jun 2011 13:12

Last Modified : 28 Jun 2011 13:12

Official URL: http://www.pjbs.org/ijps/ijps.htm

Volume 9, Number 11, - 2010 , ISSN 1682-8356

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