UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2009 > Volume 58 Number 1 > Mutsuki KURAOKA1), Takahisa FURUTA2), Takashi MATSUWAKI3), Tsutomu OMATSU1), Yoshiyuki ISHII1), Shigeru KYUWA1) and Yasuhiro YOSHIKAWA1)

Direct Experimental Occlusion of the Distal Middle Cerebral Artery Induces High Reproducibility of Brain Ischemia in Mice

Mutsuki KURAOKA1), Takahisa FURUTA2), Takashi MATSUWAKI3), Tsutomu OMATSU1), Yoshiyuki ISHII1), Shigeru KYUWA1) and Yasuhiro YOSHIKAWA1)
1) Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, University of Tokyo 2) Department of Microbiology and Immunology, The Institute of Medical Science, University of Tokyo 3) Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, University of Tokyo
Abstract :

Several investigators have used murine models to investigate the pathophysiology of brain ischemia. The focal ischemic model is a closer approximation to human stroke which includes a necrotic core, penumbra, and undamaged tissue. Occlusion of a unilateral artery, especially the middle cerebral artery (MCA), is performed in this model, but collateral circulation often induces variation of ischemic lesions both qualitatively and quantitatively. It is likely that the more proximal the artery which is unilaterally occluded is, the more inconsistent the outcomes. The present study was designed to examine the reproducibility of infarct lesion by distal or proximal artery occlusion. Direct occlusion of the distal MCA was performed and compared with unilateral common carotid artery occlusion (CCAO) in C57BL/6 mice. Direct MCA occlusion (MCAO) consistently induced ischemic lesions in cortical areas. All model animals (n=14) survived 24 h after occlusion, and exhibited a maximum infarct volume (20.0 ± 5.0%). In contrast, permanent and transient unilateral CCAO models had mortality rates of 62.5 and 25.0%, and showed severe to absent lesions with the infarct volumes of 29.0 ± 20.8 and 33.2 ± 24.2%, respectively. In conclusion, distal MCAO produces high reproducibility of ischemic insults and survivability compared to unilateral CCAO. Thus, distal MCAO is a useful method for the focal ischemic model.

Keywords :
common carotid artery occlusion, middle cerebral artery occlusion, mouse

Date Deposited : 04 Jul 2011 09:51

Last Modified : 04 Jul 2011 09:51

Official URL: http://www.jstage.jst.go.jp/browse/expanim/_vols

Volume 58, Number 1, - 2009 , ISSN 1881-7122

Download:
Full Text Original
Abstract : pdf doc
Home | Peta Situs | Admissions | Student Research | E-Journal
Universitas Muhammadiyah Malang © 2011
Developed by Infokom UMM
Last Update : 13 December 2016 20:46Kumpulan file jurnal