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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2011 > Volume 58 Number 1 > Mikako SAITO1)2), Toshiya SASAKI1) and Hideaki MATSUOKA1)2)

Vitamin B12 Promotes Cx40 and HCN4 Gene Expression at an Early Stage of Cardiomyocyte Differentiation

Mikako SAITO1)2), Toshiya SASAKI1) and Hideaki MATSUOKA1)2)
1) Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology 2) CREST (Core Research for Evolutional Science and Technology), Japan Science and Technology Agency
Abstract :

 Vitamin B12 (VB12) was investigated for its ability to influence the expression of genes required for cardiac cell differentiation from mouse embryonic stem cells. When VB12 (0.5 mM) was added to the medium on day 3 of culture, the levels of Cx40 and HCN4 expression increased over those in the control, 2 to 3 days before the start of cardiomyocyte beating. In contrast, the expression levels of α-MHC, MLC-2a, and MLC-2v were almost the same as those of the control throughout the culture period. Our results suggest that VB12 is involved in the promotion of the intercellular conducting system rather than the generation of contractile proteins.

Keywords :
cardiomyocytes, stem cells, vitamin B12

Date Deposited : 04 Jul 2011 10:02

Last Modified : 04 Jul 2011 10:02

Official URL:

Volume 58, Number 1, - 2011 , ISSN 1881-7122

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