UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


International Journal of Medical Sciences

.: Home > International Journal of Medical Sciences > 2011 > Volume 8 Number 4 > Shi Tan1, Ling Zhang1ƒx, Xiao-Ming Zhong1, Zai-Lin Yang2, Liu-Yang Zhao1, Yu-Jie Gao1, Hui-Yuan Shao1, Feng-Xian Qin1, Xian-Chun Chen1, Hui-Juan Zhang1, Hui Chen3, Li Wang

Monoclonal Antibodies against Nucleophosmin Mutants: Potentials for the Detection of Acute Myeloid Leukemia

Shi Tan1, Ling Zhang1ƒx, Xiao-Ming Zhong1, Zai-Lin Yang2, Liu-Yang Zhao1, Yu-Jie Gao1, Hui-Yuan Shao1, Feng-Xian Qin1, Xian-Chun Chen1, Hui-Juan Zhang1, Hui Chen3, Li Wang
1. Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Department of Laboratory Medicine, Chong-qing Medical University, Chongqing 400016, China 2. Center for Hematology, Southwest Hospital, Third Military Medical University, Chongqing 400016, China 3. Department of Laboratory Medicine, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China 4. Department of Hematology, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
Abstract :

Nucleophosmin (NPM1) gene mutations resulting in cytoplasmic delocalization of Nucleo-phosmin (NPMc+) are the most common genetic alteration in acute myeloid leukemia (AML). Here, we attempted to prepare monoclonal antibodies (mAbs) against NPM1 mutation A (NPM-mA) and investigated the mAbs’ clinical utility in immunohistochemical detection of NPMc+AML. The pET-32a-NPM-mA vector with the whole open reading frame of the NPM-mA gene was constructed. E.coli BL21 transformed with the vector were induced to express the NPM-mA recombinant protein. BALB/c mice were immunized with the recom-binant NPM-mA. Positive clones were selected by indirect ELISA and the mAbs were ob-tained. Immunohistochemistry was performed to detect the NPMc+ in bone marrow smears from 10 AML patients with NPM-mA. The results showed that the pET-32a-NPM-mA vector was successfully constructed and the NPM-mA recombinant protein was used to immunize the mice. Two positive clones (2G3 and 3F9) were selected. The mAbs against NPM-mA were raised, but did cross-react with wild type NPM1. The mAbs can be used to detect the cyto-plasmic dislocation of NPM1 in all AMLs carrying NPM-mA. Our results show that an-ti-NPM-mA mAbs were produced. Though they would cross-react with wild type NPM1, the mAbs may still have potential in the detection of NPMc+AMLs.

Keywords :
acute leukemia, nucleophosmin mutants, recombinant protein, monoclonal antibody

Date Deposited : 04 Jul 2011 11:15

Last Modified : 04 Jul 2011 11:15

Official URL: http://www.medsci.org/

Volume 8, Number 4, - 2011 , ISSN 1449-1907

Download:
Full Text Original
Abstract : pdf doc