Animal Bioresource in Japan
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Transgenic Mouse Sperm that Have Green Acrosome and Red Mitochondria Allow Visualization of Sperm and Their Acrosome Reaction in Vivo
Hidetoshi HASUWA 1), Yuko MURO ), Masahito IKAWA 1), Noriko KATO 2) 3), Yoshihide TSUJIMOTO 2) and Masaru OKABE 1)
1) Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, 2) Laboratory of Molecular Genetics, Department of Medical Genetics, Osaka University Medical School, 3) JCL Bioassay Corp.
In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1- Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.
live imaging, oviduct, sperm
Date Deposited : 11 Jan 2011 14:31
Official URL: http://www.jstage.jst.go.jp/article/expanim/59/1/59_105/_article
Last Modified : 11 Jan 2011 14:31
Volume 59, Number 1, - 2010 , ISSN 1881-7122 (online), ISSN 1341-1357 (print)
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