Nature and Science

Preparation of Porcine Factor V

Qiang Zhang, Xuejun Gao, Shanshan Qian, Minyue Ma, Qingzhang Li, Yingjun Cui

Abstarc :

A rapid, simple, and reproducible method for the preparation of porcine Factor V from porcine
plasma was investigated in this study. Methods: Porcine  Factor  V was isolated by multi-techniques
including polyethylene glycol precipitation, absorption of protein on to barium citrate and DEAE-cellulose
chromatography. Results:  Porcine Factor V was identified by  SDS-PAGE. Yields and purity of  porcine
Factor  V  were 90.2 mg/L of  starting plasma and 64.4%, respectively.  Conclusion:  This preparative
procedure is simple, high yields and suitable for mass production. [Nature and Science. 2004;2(3):87-90].

Key Word :
porcine plasma; Factor V; preparation

Volume 2, Number 3, October 2004 , ISSN 1545-0740