A rapid, simple, and reproducible method for the preparation of porcine Factor V from porcine
plasma was investigated in this study. Methods: Porcine Factor V was isolated by multi-techniques
including polyethylene glycol precipitation, absorption of protein on to barium citrate and DEAE-cellulose
chromatography. Results: Porcine Factor V was identified by SDS-PAGE. Yields and purity of porcine
Factor V were 90.2 mg/L of starting plasma and 64.4%, respectively. Conclusion: This preparative
procedure is simple, high yields and suitable for mass production. [Nature and Science. 2004;2(3):87-90].