Two experiments were conducted to evaluate the effects of slow-releaseurea (SRU) versus feed-grade urea on ruminal metabolite characteristicsin steers and DMI, gain, and G:F in growing beef steers. Experiment1 used 12 ruminally cannulated steers (529 ± 16 kg ofBW) to monitor the behavior of SRU in the ruminal environment.Compared with feed-grade urea, SRU decreased ruminal ammoniaconcentration (P = 0.02) and tended to increase ruminal ureaseactivity (P = 0.06) without affecting ruminal VFA molar proportionsor total concentrations (P > 0.20). After 35 d of feeding,the in situ degradation rate of SRU was not different betweenanimals fed urea or SRU (P = 0.48). Experiment 2 used 180 Angus-crosssteers (330 ± 2.3 kg) fed corn silage-based diets supplementedwith urea or SRU for 56 d to evaluate the effects on feed intake,gain, and G:F. The design was a randomized complete block witha 2 x 4 + 1 factorial arrangement of treatments. Treatmentsincluded no supplemental urea (control) or urea or SRU at 0.4,0.8, 1.2, or 1.6% of diet DM. Over the entire 56 d experiment,there were interactions of urea source x concentration for gain(P = 0.04) and G:F (P = 0.01) because SRU reduced ADG and G:Fat the 0.4 and 1.6% supplementation concentrations but was equivalentto urea at the 0.8 and 1.2% supplementation concentrations;these effects were due to urea source x concentration interactionsfor gain (P = 0.06) and G:F (P = 0.05) during d 29 to 56 ofthe experiment. The SRU reduced DMI during d 29 to 56 (P = 0.01)but not during d 0 to 28, so that over the entire experimentthere was no difference in DMI for urea source (P = 0.19). Thesecollective results demonstrate that SRU releases N slowly inthe rumen with no apparent adaptation within 35 d. Supplementationof SRU may limit N availability at low (0.4%) concentrationsbut is equivalent to urea at 0.8 and 1.2% concentrations.