International Journal of Poultry Science

Clostridium perfringens Type A from Broiler Chicken with Necrotic Enteritis

Arunava Das1, Yahya Mazumder2, Biman Kumar Dutta3, Bibek Ranjan Shome4, Komal Molla Bujarbaruah5 and Ashok Kumar6

Abstarc :

This paper reports the investigation of necrotic enteritis (NE) in six broiler chickens of age two to three weeks old, died in Jowai poultry farm, Meghalaya, India. Initially, scanning electron microscopy (SEM)
was performed to observe the morphological changes within the intestine. Intestinal contents and liver
samples from dead chicken were investigated for isolation of bacteria and their virulence determinant. The
SEM analysis of infected intestine revealed massive necrosis and complete destruction of the intestinal villi
within the intestinal mucosa. Bacterial isolation confirmed the causative agent as C. perfringens in NE. All
the isolates harboured single and double plasmid deoxyribonucleic acid with identical 45.2kb common
plasmid. In polymerase chain reaction (PCR) assay all 10 clinical isolates harboured alpha toxin gene (cpa) of C. perfringens, however, four isolates also carried additional beta2 toxin gene (cpb2). None of the isolates were positive for beta, epsilon, iota and enterotoxin genes. PCR analysis revealed that all isolates derived from NE belonged to C. perfringens type A. The partial cpa gene sequence analysis showed 97.6 to 100% homology among the C. perfringens isolates. The study confirmed that C. perfringens type A is the most predominant one associated with necrotic enteritis in broiler chickens in Meghalaya, India and the alpha toxin (CPA) might play a significant role in the pathogenesis of the disease in broiler chicken.

Key Word :
Clostridium perfringens, alpha toxin, plasmid, polymerase chain reaction, necrotic enteritis

Volume 7, Number 6, - 2008 , ISSN 1682-8356