International Journal of Medical Sciences

Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells

Xue-Wen Huang, Rui-Hua Luo, Qi Zhao, Zhong-Ze Shen, Li-Li Huang, Xian-Yuan An, Lan-Jing Zhao, Jie Wang, Yu-Zheng Huang

Abstarc :

To investigate the role of ROS in the helicobacter pylori (Hp) induced mtDNA mutations, AGS cells were treated by extracts of Hp11638 or Hp11638M. The ROS levels, cytochrome C reductions, and intracellular ATP levels were measured. The coding region and the D-Loop region were amplified and sequenced. Results showed the ROS levels, cytochrome C reduction and mtDNA mutations were markedly increased and cell viability decreased after treatment with both Hp extracts, and 616 mutations were detected in D-Loop region and 3 heteroplasmic point mutations in the Cytb gene. No mutations were found in the coding region. The mutation rates of mtDNA D-Loop region were positively correlated with the ROS levels and negatively to the ATP levels.

Key Word :
Helicobacter pylori, Reactive Oxygen Species, Mitochondrial DNA, Mutation

Volume 8, Number 1, - 2011