Nature and Science

Optimization of Asparaginase Production by Pseudomonas aeruginosa Using Experimental Methods

R. Manikandan1, CN Pratheeba2, Pankaj Sah3 and Stuti Sah 4

Abstarc :

Abstract: Evaluation  of  fermentation  process  parameter  interactions  for  the  production  of  l-asparaginase  by Pseudomonas aeruginosa. Box-Behnken  design  of  experimentation was  adopted  to  optimize  nutritional  sources, physiological  (incubation period)  and microbial  (inoculum  level). The  experimental  results  and  software predicted enzyme  production  values were  comparable.  Incubation  period,  inoculum  level  and  nutritional  source  (soybean) were major influential parameters at their individual level. Interaction data of the selected fermentation parameters could  be  classified  as  least  and most  significant  at  individual  and  interactive  levels. All  selected  factors  showed impact on l-asparaginase enzyme production by  this isolated microbial strain either at the  individual or interactive
level.  Incubation  temperature,  inoculums concentration,  and nutritional  source  (soybean) had  impact at  individual level.  Significant  improvement  in  enzyme  production  by  this  microbial  isolate  was  noted  under  optimized environment. [Nature and Science. 2010;8(2):1-6]. (ISSN: 1545-0740).

Key Word :
box-Behnken; pseudomonas aeruginosa; L- asparaginase; response surface

Volume 8, Number 2, February 2010 , ISSN 1545-0740