International Journal of Medical Sciences




Cell Cycle Arrest by a Natural Product via G2/M Checkpoint

Sharon Chui-Wah Luk, Stephanie Wing-Fai Siu, Chun-Kit Lai, Ying-Jye Wu, Shiu-Fun Pang

Abstarc :

CKBM is a natural product that exhibits a novel anti-tumor activity through the induction of cell cycle arrest and apoptosis. We have investigated its effects on cell cycle regulation using a gastric cancer cell line, AGS. The effects of CKBM on cell proliferation, cell cycle regulation and apoptosis were analyzed using BrdU (5-bromo-2'-deoxyuridine) cell proliferation assay and flow cytometric analysis, respectively. Specific cellular protein expressions were measured using
Western blot analysis. Flow cytometric analysis indicated that CKBM induced G2/M cell cycle arrest and apoptosis, whereas differential protein expressions of p21, p53 and 14-3-3َ (stratifin) using Western blot analysis were enhanced. The differential expressions of p21, p53 and 14-3-3َ in AGS cancer cells after CKBM treatment may play critical roles in the G2/M cell cycle arrest that blocks cell proliferation and induces apoptosis.




Key Word :
14-3-3َ (stratifin), G2/M arrest, cell proliferation, checkpoint protein

Volume 2, Number 2, - 2005 , ISSN 1449-1907