Nature and Science

Oral Vaccination of Nile Tilapia (Orechromis niloticus) Against Motile Aeromonas Septicaemia

Noor El Deen Ahmed Ismail(1), Nagwa Sad. Atta I (2 and Abd E Aziz ,Mohamed .Ahmed(3)

Abstarc :

Abstract: The present study was planned for preparation of formalin inactivated wet-packed whole cells
Aeromonas hydrophila bacterin for oral vaccination. The humeral antibody response of vaccinated Nile
tilapia (Orechromis niloticus (O. niloticus) was determined by micro-agglutination test. Moreover efficacy
of the prepared bacterin against infection with Aeromonas hydrophila was detection and calculated as a
relative level of protection. Nile tilapia (O. niloticus) immunized orally with formalin-inactivated
Aeromonas hydrophila .wet-packed while cells had low level of antibody titer reached 2 and 3 by log2 at
first and fourth week post-immunization respectively while Nile tilapia (O. niloticus) fed on minced meat
without vaccine had antibody titer reached 1 by log 2 throughout the experimental period . The relative level
of protection among Nile tilapia (O. niloticus) immunized orally were 86.8. [Nature and Science
2010;8(2):21-26]. (ISSN: 1545-0740).

Key Word :
Aeromonas hydrophila - bacterin -vaccination- humeral antibody- Nile tilapia.

Volume 8, Number 2, February 2010 , ISSN 1545-0740