Nature and Science
Probiotic Activity of L. acidophilus against Major Food-borne Pathogens Isolated from Broiler Carcasses.
Sherein* I. Abd El-Moez 1 , Ahmed F.Y. 2 , Samy A.A. 1 , Aisha R.Ali 3
C.jejuni, E.coli and S. typhimurium are the principal food borne pathogens in poultry industry. The first
experiment tested the effectiveness of different strains of Lactobacillus as in vitro as probiotic against C. jejuni, E.coli O157 and S. typhimurium Result showed that L.acidophilus isolated from colostrums of mare and goat showed the widest inhibition zone against C. jejuni, E.coli O157 and S. typhimurium strains compared to the use of L.acidophilus isolated from goat and cattle milk. The second experiment evaluate the efficiency of L. acidophilus isolated from mare colostrums showing highest in vitro inhibition activity against tested strains as in vivo probiotic against C. jejuni isolated from broiler carcasses. The result showed great inhibition of C. jejuni, E.coli O157 and S. typhimurium strains by the use of L.acidophilus in comparing to the use of antibiotics. In the second experiment; four groups of adult albino rats were used; group (1) control negative, group (2) rats orally administrated by L. acidophilus only from the start of experiment till the 14th day, group (3) rats challenged only
with C. jejuni and group (4) orally administrated by L. acidophilus from the start of experiment till the 14th day at the 7th day they were challenged with C. jejuni. Result showed that the third group showed the highest rate of reisolation of C.jejuni (0.80±0.16 from fecal swabs and 0.84±0.17 from the internal organs) as well as major pathological lesions in the tested organs in the form of granulomatus reaction in the lung tissue infiltration of inflammatory cells in the lung tissue thickening of wall of blood vessels with alveolar emphysema. Congestion hemorrhages of renal blood capillaries and coagulative necrosis of the renal tissue as well as degeneration and necrosis of hepatocytes with proliferation of fibrous tissue. The forth group pretreated with L. acidophilus Showed lower rate of isolation of C.jejuni (0.08±0.02 from fecal swabs and 0.04±0.01 from internal organs. The pathological
findings of the internal organs showed minor lesions in the form of interstitial pneumonia and inflammatory cellular
infiltration in the lung Swelling and degeneration of renal epithelium and hepatocytic degeneration with infiltration
of inflammatory cells. The second group which was only treated with L.acidophilus showed no reisolation of
C.jejuni as well as no pathological lesions were detected except a minor lesion in the liver in the form of diffused
vacuolar degeneration in hepatocytes. Results develop a safe method for competing food borne pathogens in edible
animals and suggest the need for probiotics to hinder the spread of highly pathogenic zoonotic bacteria transmitted
by animal food by products. [Nature and Science 2010; 8(3):69-78]. (ISSN: 1545-0740).
Key Word :
C. jejuni; L. acidophilus; probiotics; in vivo, antibiotic sensitivity, rat.
Volume 8, Number 3, March 2010 , ISSN 1545-0740