The Prison population is at risk of HIV infection even though they are most often neglected risk group in the area of prevention and management. The seroprevalence of Human Immunodefiency Virus [HIV] infection among male inmates in Kuje prison, Abuja- Nigeria was determined. Two hundred sera specimens from the prison inmates were tested using 3 different test kits. Of 200 samples tested 12 [6%] had HIV-1 antibodies. The highest prevalence of HIV antibodies was found in the age group of 10-20 years [7.1%]. This was followed by the age group of 21-30 years [6.8%] while the least [4.0%] was observed among those aged 41-50 years. HIV among inmates particularly the young was high. Preventive and management measures should always be extended to inmates of Nigerian prisons. Prisoners should continuously be educated about HIV pandemic and methods of prevention. [Nature and Science 2010;8(6):92-94]. (ISSN: 1545-0740).