Animal Bioresource in Japan

Fetal Sex Determination of Macaque Monkeys by a Nested PCR Using Maternal Plasma

Fusako MITSUNAGA1)2), Miyuki UEIWA1), Yoshirou KAMANAKA1), Mayumi MORIMOTO1) and Shin NAKAMURA1)

Abstarc :

Non-invasive fetal sex determination is required for biomedical studies, in which some sexual difference would be expected in fetal events, in order to make a choice of male or female fetus. To detect male fetal DNA of the sex-determining region Y gene (SRY) in maternal macaque plasma, nested real-time PCR using the SYBR Green system was developed. In all cases of pregnant macaques with male fetuses, a nested PCR product of SRY was amplified from the mother’s plasma, while no amplicon was detected in any case of pregnancy with a female fetus. Interestingly, fetal SRY DNA appeared to be cleared rapidly from the maternal blood after parturition. The current method is sensitive and can be performed with a regular PCR machine.

Key Word :
fetal DNA, maternal blood, SRY

Volume 59, Number 2, - 2010