Acetolactate decarboxylase (ALDC) gene was isolated from Bacillus subtilis by PCR
amplification. The 0.8 kb amplified fragment was confirmed to be the ALDC gene by DNA sequencing.
The gene was inserted into plasmid pYC6/C7 and transformed HD34 beer yeast, as well as 2008
transformants were obtained. [Nature and Science. 2004;2(3):84-86].