Nature and Science

Acetolactate Decarboxylase from Bacillus subtilis: Cloning and Transformating in HD34 Yeast

Wenxiang Ping, Jingping Ge, Zhuangwei Lou, Gang Song

Abstarc :

Acetolactate  decarboxylase  (ALDC) gene was  isolated from  Bacillus subtilis by PCR
amplification. The 0.8 kb amplified fragment was confirmed to be the ALDC gene by DNA sequencing.
The  gene was inserted into plasmid pYC6/C7 and transformed HD34  beer yeast,  as well as  2008
transformants were obtained. [Nature and Science. 2004;2(3):84-86].

Key Word :
Bacillus subtilis; acetolactate decarboxylase; gene cloning; transformation

Volume 2, Number 3, October 2004 , ISSN 1545-0740